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High content imaging (HCI, also called High Content Screening) describes an automated workflow that can be divided into three-steps: image acquisition, image processing and data analysis. HCI can massively increase the throughput of imaging experiments, reducing user bias and improving the quantitation of image data. HCI can be used in large-scale assays such as CRIPSR screens and drug library screens.

The Facility has a Molecular Devices ImageXpress HT.ai which is a confocal and wide-field imaging system specifically designed to image cells cultured in standard format multi-well plates (although it can also image cells on slides).  The HT.ai is equipped with a robotic plate loader which significantly increases the throughput capability of the system.  Potential applications for this system include live/dead analysis, cell cycle analysis, apoptosis, cytoplasmic/ nucleus translocation assays, DNA damage, tube formation (angiogenesis), neurite outgrowth, micronucleus assays, morphometric analysis (tubulin) and cell migration.

Our System: